Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jul 17;11(5):1117–1128. doi: 10.1111/j.1582-4934.2007.00081.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

PMC Copyright notice

Purification of IgG1 and IgG4 autoantibodies specific to the epidermal basement membrane. IF microscopy study of serum from patient 1 on normal human skin demonstrates deposition of mainly IgG1 (A) and IgG4 (D) and traces of IgG2 (B) and IgG3 (C) at the dermal–epidermal junction. After purification against an affinity matrix containing mon-oclonal antibodies specific for IgG1, IgG1 autoantibodies to the skin basement membrane can be visualized in the purified fraction (E), while IgG2 (F), IgG3 (G) and IgG4 (H) autoantibodies are not detectable. Conversely, the antibody fraction purified against an affinity matrix specific for IgG4 demonstrated binding of autoantibodies to the basement membrane of normal human skin exclusively belonging to the IgG4 subclass (L), whereas no deposits of IgG1 (I), IgG2 (J) or IgG3 (K) were observed (all magnifications, ×200).