Bead control	Capture experiment	Competition control
Protein extract (10 mg/ml)	30 µl	30 µl	30 µl
c-di-GMP (10 mM)	0 µl	0 µl	10 µl
Nucleotides (10 mM of each nucleotide)	10 µl	10 µl	10 µl
Capture buffer 5x	20 µl	20 µl	20 µl
H2O	42 µl	32 µl	22 µl
30 min incubation
c-di-GMP~CC (stock 100 µM)	0 µl	5-10 µl	5-10 µl
Final concentrations:	Bead control	Capture experiment	Competition control
c-di-GMP~CC (µM)	0 µM	5-10 µM	5-10 µM
Competitor c-di-GMP (µM)	0 µM	0 µM	1,000 µM