Table 1.
Data collection and refinement statistics
| 70S-pYa | 70S-mRNA-tRNAsa | |
|---|---|---|
| Data collection | ||
| Space group | P212121 | P212121 |
| Cell dimensions | ||
| a, b, c (Å) | 209.16, 448.37, 618.12 | 209.70, 450.05, 624.09 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
| Resolution (Å) | 309-2.30 (2.36-2.30) | 312-2.50 (2.56-2.50) |
| R merge | 15.1 (186.6) | 14.1 (111.6) |
| I / σI | 8.50 (0.85)b | 8.27 (0.90)c |
| Completeness (%) | 97.6 (81.4) | 97.9 (88.5) |
| Redundancy | 5.60 (4.70) | 3.28 (2.52) |
| Refinement | ||
| Resolution (Å) | 173.22-2.32 | 122.01-2.50 |
| No. reflections | 2.467.089 | 1.962.003 |
| Rwork / Rfree | 20.69/24.67 | 23.07-28.07 |
| No. atoms | ||
| Protein | 93.016 | 90.976 |
| Ligand/ion | 194.892 | 203.092 |
| Water | 9.516 | 5.058 |
| B factors | ||
| Protein | 59.5 | 62.8 |
| Ligand/ion | 54.9 | 59.1 |
| Water | 43.8 | 46.3 |
| r.m.s. deviations | ||
| Bond lengths (Å) | 0.006 | 0.006 |
| Bond angles (°) | 1.151 | 1.063 |
Values in parentheses are for highest-resolution shell.
a
Single crystal was used to obtain the structure
b
I/σI = 2 at 2.5Å resolution
c
I/σI = 2 at 2.7Å resolution