Fig 6. The N15D mutation favors the dissociation of HsTIM.

The stability of HsTIM dimers was tested by incubating enzymes (500 μg/mL) at 30°C for 3 hours and then loading them onto a size-exclusion chromatography column (Superdex 75) equilibrated and developed in 50 mM Tris pH 8.0 plus 150 mM NaCl and 5% (v/v) glycerol. For simplicity, only the data for the WT, the N15D and the N15D/N71D proteins are shown. The profile for the N71D mutant overlaps with that of the WT enzyme.