Figure 1.
Colonization of S. cristatus in murine oral cavities. The plaque samples were taken from tooth surfaces and surrounding gingival mucosa 7 days after the last inoculation of S. cristatus in the oral cavities. DNA extracted from these plaque samples (50 μl final volume/sample in TE buffer) was examined for quantitation of S. cristatus using qPCR with primers corresponding to 23S rDNA as probes. ANOVA analysis revealed no significant difference in colonization efficiencies between the wild-type CC5A and the knockout mutant ArcAE (p > 0.05). Data represent the means ± S.D. from 5 mice per group in Experimental Groups 3, 4, and 5.