Skip to main content
. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Sci Signal. 2015 Apr 7;8(371):rs2. doi: 10.1126/scisignal.2005966

Fig. 1.

Fig. 1

Radioimmunoprecipitation assay (RIPA) buffer solubilizes many, but not all, cellular proteins. (A) Examples of proteins that are entirely solubilized (100% in the supernatant, Sup). (B) Examples of proteins that are mostly solubilized (>90% Sup). (C) Examples of proteins that are partially solubilized (≤90% Sup). (D) Dimethyl-lysine 4 histone H3 (H3K4me2) resides almost entirely in the RIPA-insoluble pellet (Pel). Band intensities were quantified from the 16-bit digital image by densitometry in ImageJ and are shown normalized to the Sup lane for each target. n.d., not detected. Data are representative of 2–4 experiments.