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. Author manuscript; available in PMC: 2015 Apr 17.
Published in final edited form as: Clin Cancer Res. 2009 Feb 15;15(4):1210–1221. doi: 10.1158/1078-0432.CCR-08-1270

Figure 5. Defibrotide (DF) treatment suppresses the expression and function of heparanase.

Figure 5

RT-PCR was performed as described in “Material and methods”. Results are expressed as mean mRNA heparanase level normalized by β-actin housekeeping gene. DF treatment suppresses the levels of heparanase transcript in RPMI-8226 (panel A) MM cells. Furthermore, DF suppresses the intracellular protein expression of heparanase (measured by flow cytometric analysis) (panel B); as well as the heparanase activity in cellular extracts of RPMI-8226 (panel C) MM cells. Student test: *p< 0.05 and **p<0.01.