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. 2015 Apr 17;10(4):e0125174. doi: 10.1371/journal.pone.0125174

Fig 9. GmGATA44 modulates chlorophyll content.

Fig 9

(a) Expression levels of GmGATA44 and AtGNC in the wild-type Arabidopsis (wt), the gnc mutant and two GmGATA44 overexpressing transgenic lines (OX31 and OX43) using semi-quantitative RT-PCR from 3 week old rosette leaf tissue.(b) Images of the wild-type plant, the gnc mutant and GmGATA44 overexpressing transgenic plants at one week (upper panel), 3 weeks (middle panel) and 5 weeks (bottom panel) post germination. Bars = 1 cm.(c) Chlorophyll content of the wild-type plants, the gnc mutant and two GmGATA44 overexpressing transgenic lines at 3 weeks post germination. Data are presented as mean ± SD (N = 10) from triplicate independent measurements. Data analysis was performed using SAS software, and significant differences were calculated using the Student’s t-test at 95% confidence limit. Asterisk indicates significant differences from the wild-type plant.(d) Relative expression levels of AtPORA, AtPORB and AtPORC in the wild-type plant, the gnc mutant and two GmGATA44 overexpressing transgenic lines by real-time PCR from 3 week old rosette leaf tissue. Data were obtained by real-time PCR normalized against the reference gene GAPDH and shown as a percentage of expression in the wild-type plant.