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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Nature. 2015 Jan 26;520(7547):378–382. doi: 10.1038/nature14044

Extended Data Fig. 8. Inhibition of bovine Pin1 does not affect Theileria-associated cell transformation.

Extended Data Fig. 8

a. Buparvaquone (Bup), Juglone (Jug) and DTM decreased the viability of cells infected with T. parva (TpMD409). Host cell viability was assessed using the XTT assay after 72h.

b. Colony formation of parasite-infected TpMD409 cells in soft-agar was lost after 72h treatment with Buparvaquone (Bup), Juglone (Jug) or DTM. Number of macroscopic colonies per plate were counted after 10 days.

c. siPin1 has no effect on TBL3 transformed phenotypes. TBL3 were transiently transfected with siControl or siPin1. The average number of colonies per plate is shown. (average ± sd, n=3). Indicated protein levels were detected by Western blot analysis using specific antibodies. Actin was used as loading control (Results representative of 3 independent experiments).

d/f. Overexpression of the TaPin1 Mutant A53P rescued the Buparvaquone but not Juglone effects on Theileria infected cells. All data represent 3 independent experiments (average ± sd, n=3). The SPSS 19.0 program (SPSS Inc. Chicago, IL, USA) was used for statistics. *p<0.05, **p<0.01.