Skip to main content
. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Nature. 2015 Jan 26;520(7547):378–382. doi: 10.1038/nature14044

Extended Data Fig. 5. TaPin1 functionally replaces hPin1.

Extended Data Fig. 5

a. The HA-tagged version of TaPin1 and hPin1 can rescue cell spreading defects in knockout pin1-/- murine immortalized fibroblats. Cell spreading was assessed by Phalloïdin-TRITC staining. “Con” indicates Control = transfection with the appropriate empty vector.

b. TaPin1 causes centrosome amplification. NIH3T3 fibroblasts stably expressing TaPin1 or hPin1 were arrested at the G1/S transition by aphidicolin, stained with anti-γ-tubulin antibody (arrow) and counterstained with DAPI.

These photos are representative of cell in 3 independent experiments