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. 2015 Apr 17;10(4):e0123659. doi: 10.1371/journal.pone.0123659

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© 2015 Yang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — On day 14 following the second immunization, mice were sacrificed and single-cell suspensions were prepared from the spleen, cultured for 48 h, and stimulated with 5 μg/mL purified Ah01/AA viral antigen. IFN-γ (A) and IL-4 (B) secretion by splenocytes was determined by ELISPOT in triplicate wells. Values and bars represent means ± SD. ** p<0.001 compared to the PBS group.