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. Author manuscript; available in PMC: 2015 Oct 16.
Published in final edited form as: Nature. 2015 Jan 26;520(7547):383–387. doi: 10.1038/nature14100

Extended Data Figure 4. Mass spectrometric comparison of apo to holo conversion of PCP4 with Sfp and D-pHPG-L-Ser-S-coenzyme A (4) forming D-pHPG-L-Ser-S-PCP4 (5).

Extended Data Figure 4

Top: mass spectrum (ESI+) of apo-PCP4. Bottom: mass spectrum (ESI+) of D-pHPG-L-Ser-S-PCP4 (5) derived from treatment of apo-PCP4 with Sfp and corresponding synthetic dipeptidyl-CoA substrate 4. Owing to diketipiperazine22,31 formation and slow hydrolysis to the unloaded holo-PCP4, the half-life of 4 is approximately 20 min.