Extended Data Figure 3. Angiotensin receptor AT1 is enriched in ETV-1+ neurons in the SFO.

Quantitative PCR analysis of gene expression in three groups of neurons: ETV-1+ neurons from the SFO, ETV-1+ from the cerebral cortex³¹, and Vgat+ neurons from the SFO. Individual data points were normalized to the levels of GAPDH. Shown are the qPCR results for ETV1, nNOS, Vgat and AT1 in the three different samples; data is presented for each gene as its relative abundance compared to the tissue with the lowest level of expression (for example nNOS is expressed 10x more abundantly in ETV1-positive neurons from the cortex than in Vgat neurons from the SFO, and 1000x more abundantly in ETV-1 positive neurons from the SFO than the cortex). Note, that AT1 is highly enriched in ETV-1+ SFO neurons. N.D.: not detected. Values are means ± s.e.m (n=3 technical replicates)