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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Microsc. 2015 Jan 22;258(2):105–112. doi: 10.1111/jmi.12220

Figure 2.

Figure 2

Characterization of various imaging techniques. (A) Brightfield and (B) DICM images of the same region of the intestine of a 7 dpf (days post fertilization) larval zebrafish. In the DICM image, features such as the gut edge (arrows) are clearly evident. (C) Pixel intensity, normalized to a peak value of 1, as a function of depth relative to the focal plane from images of a fluorescent polystyrene microsphere embedded in agar imaged with BF (brightfield), DICM, and LSFM, providing a measure of the depth of focus, and optical sectioning ability, of each method.