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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Clin Cancer Res. 2015 Jan 21;21(8):1962–1972. doi: 10.1158/1078-0432.CCR-14-2616

Figure 5. Preference for NHEJ of mutant and complemented FA HNSCC cells.

Figure 5

A) Schematic of DNA repair reporter assay constructs for NHEJ. B) Reporter assays performed on isogenic VU-1131 cells indicate that FA gene correction decreases baseline preference for NHEJ. ** p < 0.01 (t-test). C) Immunoblot of total DNA-PKcs and pDNA-PKcsS2056 in FAmut and FAcomp cell lines treated with 2 μg/mL bleomycin for 20 minutes in the presence and absence of 24 hours pre-treatment with the DNA-PKcs inhibitor NU-7026 (2 μM). Pretreatment with NU-7026 decreases phosphorylation of DNA-PKcs caused by bleomycin treatment. D) Chemical inhibition of DNA-PKcs does not decrease the cisplatin EC50 of FAmut HNSCC cells relative to FAcomp cells following 2 days of exposure.