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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Cancer Res. 2015 Mar 5;75(8):1592–1602. doi: 10.1158/0008-5472.CAN-14-1493

Figure 6. Blockade of DLL and JAGGED ligands promotes liver metastases.

Figure 6

(A) N11–24-decoy containing EGF repeats 1–24, blocks DLL and JAGGED ligands; N11–13-decoy containing EGF repeats 1–13, blocks DLL ligands; and N110–24-decoy containing EGF repeats 10–24, blocks JAGGED ligands. (B) Quantification of total flux (photon/sec) by ex vivo liver bioluminescence 3 weeks after intracardiac injection of 106 cells for NGP-Fc (n=10), NGP-N11–24-decoy (n=10), N11–13-decoy (n=10), N110–24-decoy (n=10). *P<0.05; ***P<0.001. (C) Diameter of liver metastasis, with each point on the scatter plot an individual metastasis. N11–24-decoy (n=87, from 7 tumors) and N11–13-decoy (n=72, from 6 tumors). *P<0.05. (D) Liver metastases were immunostained for ICAM-1 (upper panel). Mean±stddev. P=n.s. Bar, 100μm. αSMA (middle panel). Mean±stddev. *P<0.05. Bar, 100μm. Double immunostaining (bottom panel) for desmin (green) and αSMA (red), demonstrated co-localization (arrows), inset (2x magnification). Bar, 50μm.