Fig 2. Recombination expression vector of pB7FWG2-GmCnx1 and determination of GmCnx1 transgenic soybean plants.

(A) Vector contained selective maker gene (bar) coded phosphinothricin acetyltransferase (PAT) and showed resistance to the herbicide phosphinothricin, green fluorescent protein gene (Egfp) and GmCnx1 gene. LB, left border; RB, right border; p35s, promoter; T35S, terminator. (B) T₁ transgenic lines were confirmed by coating leaves with Phosphinothricin. (C)T₁ transgenic lines were confirmed by bar protein quick dip strip. (D) T₁ transgenic lines were confirmed by PCR. (E) Southern blot analysis of transgene copy number in T₁ transgenic soybean and WT. Genomic DNA and plasmid DNA was digested with restriction enzyme EcoR Iand HindⅢ. The probe was used for GmCnx1. M, DL2000 marker; +, positive control (plasmid DNA); WT, negative control, non-transgenic plants; 0, blank. L1, L2, L3, L4, L7, L10, L17, L18, L22 and L26 represent the GmCnx1 transgenic line numbers.