Fig 3. p53R172H is required for transformation-related phenotypes in HCC cell lines.
A) Immunoblot of p53 depicting 3 HCC cell lines infected with shRNA as compared to an empty vector control labeled pGIPZ. B) Transwell migration assay of p53 shRNA-infected HCC cell lines. A p53-null cell line was used as an experimental control. p-values, calculated by Student’s t-test, are 0.020 and 0.022 for R172H lines 1 and 2, respectively. The number of migrating cells in each pGIPZ control infection was set to 100%. C) Soft agar colony formation assay of p53 shRNA-infected HCC cell lines. A p53-null cell line was used as an experimental control. p-values, calculated by Student’s t-test are 0.044 and 0.004 for R172H lines 1 and 2, respectively. The number of colonies for each pGIPZ control infection was set to 100%.