Fig 2. Oxygen consumption and extracellular acidification rate by neutrophils.

(A) Oxygen consumption rate (OCR) and (B) extracellular acidification rate (ECAR) by neutrophils from WT, Hvcn1 ^-/- and gp91^phox-/- mice in response to stimulation with PMA (with and without DPI) or opsonised Candida. The numbers of independent experiments is shown over the total number of measurements. Statistical significance: *** p<0.001, ** p<0.01 and * p<0.05. Differences between PMA stimulated WT and gp91^phox-/- were p<0.001 in (A) and p<0.05 in (B). There were no significant differences in (B) between PMA and Candida or between WT and Hvcn1 ^-/- cells. Median, quartiles and 95% centiles are shown.