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. 2015 Apr 17;10(4):e0125906. doi: 10.1371/journal.pone.0125906

Fig 4. The effect of DPI, azide, 4ABH, KCN, zinc, and CCCP on vacuolar pH.

Fig 4

(A) The effect of 300 μM Zn2+, DPI and 60 μM CCCP on vacuolar pH in WT and Hvcn1 -/- neutrophils at ~30 minutes following the addition of Candida without synchronisation to particle uptake. (B) The effect of 5 μM DPI, 5 mM sodium azide, 50 μM 4ABH and 1mM KCN, on vacuolar pH in human neutrophils at ~30 minutes following the addition of Candida without synchronisation to particle uptake. The effect of the addition of azide (C) or DPI (D) on vacuolar pH in human neutrophils synchronised to time of addition (red arrows). The numbers of independent experiments is shown over the total number of measurements. Median, quartiles and 95% centiles are shown. Statistical significance: *** p<0.001.