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. 2015 Apr 17;10(4):e0123987. doi: 10.1371/journal.pone.0123987

Fig 3. High levels of glucose alter H3K27me3 and RNA polymerase 2 association at the miR-200b promoter region.

Fig 3

A: ChIP-qPCR analysis of H3K27 trimethylation (H3K27me3) at the miR-200b promoter region. Association of H3K27me3 at the miR-200b promoter region was significantly increased in 25mM (HG) glucose compared to 5mM (NG) glucose. B: ChIP-qPCR analysis of RNA polymerase 2 (Pol2) at the miR-200b promoter region. Association of Pol2 at the miR-200b promoter region was significantly decreased in HG compared to 5mM NG. C: ChIP-qPCR analysis of H3K27me3 at the human alpha-satelite region. Association of H3K27me3 at the human alpha satellite region showed no significant change between HG and NG. D: ChIP-qPCR analysis of Pol2 at the GAPDH promoter region. Association of Pol2 at the GAPDH promoter region showed no significant difference between 25mM (HG) glucose and 5mM (NG) glucose. Association of IgG and ascitic fluid (negative control) was minimal and showed no difference between NG and HG in all experiments. [* p < 0.05 compared to NG; n = 3; data expressed as mean percentage of input ± SEM].