Skip to main content
. 2015 Apr 17;10(4):e0125054. doi: 10.1371/journal.pone.0125054

Fig 9. The formation of TRAP-positive MNCs in M-BMMs was also inhibited by BIX02189 or XMD8-92.

Fig 9

(A, B) M-BMMs were pretreated with or without BIX02189 (A) or XMD8-92 (B) for 6 hrs and then cultured in the presence of M-CSF (10 ng/ml) and sRANKL (10 ng/ml) for 7 days. The TRAP activity in culture medium diluted ten times was measured. Results are expressed as the mean ± SD of sixplicate cultures. Significant differences were examined using the Student’s t-test, *P<0.05 compared with the culture without BIX02189 or XMD8-92. (C) The viability of the cells during the experiment was measured. Similar results were obtained in two independent experiments. (D, E) The induction of c-Fos was monitored at the mRNA and protein levels. Similar results were obtained in two independent experiments. Significant differences were examined using the Student’s t-test, *P<0.05 compared with the culture with M-CSF.