Fig 11. Stomata movement of OsPP108 overexpression transgenic lines under ABA treatment.
(A) Stomata aperture of 4 week old WT and OsPP108OX lines, L1, L2 and L4, in mock treatment (Stomata Opening Solution; SOS) (upper panel) and 100μM ABA (lower panel). 30 stomata were analyzed for each genotype and representation of those are shown in pictures (n = 3). Pictures were taken at 60x magnification in Olympus BX53 fluorescence microscope in bright field. Scale bar = 5μM. (B) Graph depicting percentage of different type of stomata (closed, partially open and open) in WT and OsPP108OX transgenic in mock and ABA treatments. Stomata were grouped based on apertures sizes, i.e., open- 2.5–5.0μM, partially open- 1.0–2.5μM and closed- < 1 μM. Percentage of stomata is calculated based on the average of three independent replicate experiments.