FIG. 2.

(a) Exploded view of assembly components in in vitro brain microvasculature model. (b) Photograph showing an extended PDMS reservoir connected to collagen microchannels. (c) Fabrication steps for brain microvascular structure embedded in collagen matrix. After the insertion of microneedles into the 3D printed frame (ii), and collagen mixture is applied in the square opening of 3D printed frame (iii). After geleation, the microneedles are slowly removed from the bulk collagen (iv), leaving behind four microchannels within the collagen matrix. (v) By seeding brain endothelial cells in the collagen microchannels, array of brain microvessels embedded within collagen is formed (vi).