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PCR amplification of samples DNA with the Entamoeba histolytica specific primers
Lane M: molecular marker (100 bp) ladders, C+: positive control (E. histolytica DNA), C−: negative control (H2O), Lane 1: amplified product (220 bp) indicating positive sample, Lanes 2–4: Positive Samples in microscopic examination that were not amplified by PCR.
