Fig. 1.

Generation and confirmation of FSP-ARKO mice. A: Genotyping indicated that Cre and floxed AR could be detected in the genomic DNA from tail snips of FSP-ARKO mice. B: Detection of the ARKO band at the RNA level. The primers located on exon 1and exon 3 of AR coding region were used to indicate the knockout of exon 2 gene by comparing PCR product size difference (WTAR band: 372 bp; exon 2 knockout band: 220 bp).C: ROSA26r-β-Gal mice were used to validate that FSP promoter drives the Cre expressed in the stromal compartment of prostate (VP, DLP, and AP). Arrows show positive cells by IHC staining, and the highest Cre activity was observed in VP. Prostate stromal cells show efficient recombination and intensive staining accordingly, and the epithelial cells have no staining signal. D: AR IHC staining (using C19 antibody) was used to validate that AR gene was deleted in the VP of FSP-ARKO mice. (Scale bar = 20 µm).