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. 2015 Jan 30;4(4):551–564. doi: 10.1002/cam4.412

Figure 1.

Figure 1

miR-221 and miR-222 suppress invasiveness in immortalized normal bronchial epithelial cells. (A) Quantitative reverse transcription PCR (qRT-PCR) analysis of miR-221 and miR-222 in HBEC4 transfected with miR-221 or miR-222 mimics. After transfection, high expression of these microRNAs was confirmed. (B) Soft agar colony formation (left) and invasion (right) assays for HBEC4 cells transfected with miR-221 or miR-222 mimics. miR-221 and miR-222 did not affect anchorage-independent growth but suppressed invasiveness in HBEC4 cells. The results are averages of three independent experiments done in triplicate wells. ***P < 0.001 (Mann–Whitney U test). Kras+p53 knockdown (KD) HBEC4 cells were generated by introducing pLenti6-KRASV12 and pSRZ-p53 21,22 vectors into HBEC4 cells and were used as positive control for the soft agar colony formation assay.