Fig 6.

In vitro ethanol tolerance experimental paradigms. Diagrams illustrate the different experimental protocols used to assess acute tolerance (top), rapid tolerance (middle), and chronic tolerance (bottom) of in vitro phase resetting. For the latter two paradigms, EtOH is initially provided to the mice to drink, while in the first paradigm, EtOH is only applied to the brain slices for varying amounts of time immediately prior to EtOH co-application with either glutamate or the 5-HT_1A,5A,7 receptor agonist, 8-OH-DPAT (DPAT). Dark bars: lights-off (in vivo), or subjective night (in vitro).