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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Methods Enzymol. 2014 Dec 26;551:285–321. doi: 10.1016/bs.mie.2014.10.059
  A-tailing mix
Component Volume/reaction (µl) Vendor Catalog number
10 mM dATP 1 Enzymatics N2010-A-L
10× Blue buffer 2 Enzymatics B0110
Klenow (3'–5' exo-) (Low concentration) 0.5 Enzymatics P7010-LC-L