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. Author manuscript; available in PMC: 2015 Apr 19.
Published in final edited form as: Biochem Pharmacol. 2014 Jul 24;91(3):369–379. doi: 10.1016/j.bcp.2014.07.013

Fig. 5.

Fig. 5

Basal phosphorylation of S892 is decreased by inhibiting PKA in hippocampal neurons of WT mice. Western blot of cultured neurons in the presence and absence of H89 (10 µM, 2 h pre-incubation; left). Inhibition of PKA does not affect the amount of GB2 protein but decreases the amount of S892 phosphorylation (GB2-pS892). β-III-Tubulin was used as a loading control. Bar graph summarizing the amount of phosphorylated S892 (GB2-pS892 in %; right). The total amount of GB2 and GB2-pS892 were normalized to β-III-tubulin on the same blot. Data are means ± SD, n = 6. *, p < 0.05; t test.