Extended Data Figure 4. NRF1 knockdown decreases the mTORC1-stimulated expression of PSM genes and protein degradation.

a, The expression levels of an additional PSM gene from the experiment in Fig. 2b is shown. *P<0.05 compared to vehicle-treated TSC2-expressing cells; ##P<0.01 compared to vehicle-treated vector-expressing cells. Data are mean ± s.e.m (n=3). b, The same experiment shown in Fig. 2b, except the littermate-derived pair of Tsc2^+/+ p53^−/− and Tsc2^−/− p53^−/− MEFs were used. Data are shown as the means ± s.e.m. (n=3). *p < 0.05 or **p < 0.01; ##p < 0.01, or ###p < 0.001. c, MEF cell lysates obtained from the experiment in Fig. 2c were subjected to immunoblotting. d, The same experiment in Fig. 2c was performed, except HeLa cells expressing non-targeting shRNAs (shLUC) or shRNAs targeting human TSC2 (shTSC2) were used. Data are presented as mean ± s.e.m. relative to vehicle-treated shLUC-expressing cells (n=3). *P<0.05, #P<0.05. e, f, Cell lysates obtained from the experiment in Fig. 2d and 2e, respectively, were subjected to immunoblotting. g, Autoradiograph of gel, representative of three independent experiments, corresponding to the data graphically represented in Fig. 2e. a,b,d, Statistical significance for pairwise comparisons evaluated with a two-tailed Student’s t test.