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. Author manuscript; available in PMC: 2015 Apr 19.
Published in final edited form as: J Immunol Methods. 2007 Feb 2;321(0):107–120. doi: 10.1016/j.jim.2007.01.005

Figure 2.

Figure 2

Umbilical cord blood cells (left) and adult PBMCs (right) are shown gated on CD3+CD4+ T cells. CD25+Foxp3− (upper left gate), CD25+Foxp3+ (upper right gate) and CD25−Foxp3+ (lower right gate) CD3+CD4+ cells were sorted into a dry pellet and frozen at −80°C prior to multiplex PCR.