(A) Predicted miR-4284 binding sites in the 3’UTR of CXCL5 with sequence complementarity and phylogenetic conservation of the 8 nt seed-sequence indicated, as predicted by miRNA databases Targetscan and microRNA.org. (B) Luciferase assays using a reporter vector (psiCHECK2) carrying the 3’UTR of CXCL5 inHEK293T cells transfected with negative control microRNA mimics (Control, 50 nM) or miR-4284 mimics (50 nM), for 36 hours. Experiments were performed in triplicates; values represent mean ±SD, **p < 0.01, t-test analysis, Prism6 (GraphPad Software Inc.). (C) Real-time PCR for endogenous CXCL5 mRNA levels in NCM460 colonic epithelial cells transfected with 50 nM mimics (miR-4284) or inhibitors of miR-4284 (a-miR- 4284), as compared to their respective negative controls. Values represent mean ±SD.