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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Immunol. 2015 Mar 27;194(9):4528–4534. doi: 10.4049/jimmunol.1402760

Figure 4. IL-17RB recruits STAT5 through tyrosine residues.

Figure 4

A) Schematic representation of IL-17RB with six tyrosine residues within the intracellular domain. B) KECs were left uninfected or infected with Adenovirus encoding IL-17RB followed by IL-25 stimulation. Cell lysates were incubated with anti-p-Tyr antibodies followed by immunoblot analysis. C) HEK293 cells were transfected with 1μg each of constructs expressing V5-tagged IL-17RB or V5-tagged IL-17RB all Tyr, and STAT5. Cell lysates were immunoprecipitated with anti-V5 antibody followed by western blotting analysis for STAT5 and V5. Co-immunoprecipitated products, top; Whole cell lysate (WCL), bottom. Results shown are representative data of 3 independently performed experiments.