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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Pain. 2015 May;156(5):825–836. doi: 10.1097/j.pain.0000000000000120

Figure 1. ROS scavenger (tempol) inhibits vocalizations in a visceral pain model.

Figure 1

Bar histograms show the duration (mean ± SE) of audible (A,C) and ultrasonic (B,D) vocalizations evoked by brief (10 s) colorectal distension (CRD) with innocuous (20 mmHg) and noxious (80 mmHg) intraluminal pressure (see Methods). A,B, Stereotaxic application of a ROS scavenger (tempol, 100 mM, concentration in microdialysis probe, 15 min) into the amygdala (CeA) had no effect on vocalizations of normal rats (“control”) but inhibited the increased vocalizations 5 h after intracolonic zymosan (“colitis”) significantly (n = 5 rats). C,D, Off-site application of tempol into the adjacent striatum had no significant effect (n = 5 rats). *,**,*** P < 0.05-0.001 compared to normal (control); #,## P < 0.05, 0.01, compared to predrug baseline vocalizations measured in the presence of ACSF (vehicle control; see Methods), Bonferroni posttests following repeated-measures ANOVA.