(A) Survival of C57BL/6J (black, n=27), μMT (red, n=30), J558 Tg (blue, n=13) or SMB19 Tg mice (green, n=29) infected with A.f. i.v. Results are pooled from 3 experiments with similar results. Asterisks denote significant differences in average survival rate of SMB19 Tg mice compared to J558 Tg, μMT, and C57BL/6J groups. (B-D) Serum concentration of SMB19 idiotype-specific IgM(B), GBSIb type-specific IgM (C), or anti-α-1,3 glucan-specific IgM (D) in C57BL/6J, SMB19 Tg, J558 Tg, or μMT mice at various time points after i.v. infection with A.f. n=3 / group. Bar colors correspond to mouse genotypes in Fig 5A. No serum antibodies were detected in μMT mice (n.d., red bars). Results are representative of 2 independent experiments with similar results. Asterisks denote significant differences in serum antibody levels between mouse groups at various time points. Data was analyzed using a One-way ANOVA with a Tukey’s post test. In survival studies, statistical significance was determined by log rank test, *= p<0.05, **= p<0.0, ***= p<0.001, ****; p<0.0001.