ELISPOT analysis of (A) dsDNA-, (B) histone-, and (C) nucleosome-specific splenic AFCs in 6–8 mo old B6, B6.Sle1b and Sle1b-BAC90 mice. Total IgM (D) and IgG (E) serum titers at 6–8 mo of age in each indicated group of mice. (F–K) ELISA analysis of IgG2c specific antibodies against dsDNA, histone, nucleosome, Sm/RNP, cardiolipin as well as IgG2b specific antibodies against nucleosome at 6–8 months of age. (L–N) ELISPOT analysis of dsDNA-, histone-, and nucleosome-specific long-lived bone marrow AFCs in 6–8 mo old mice of indicated genotypes. Each circle represents an individual mouse. *p <0.05, **p <0.01, ***p <0.001.