(A–E) ELISPOT analysis of IgM-, IgG-, dsDNA-, histone-, and nucleosome-specific splenic AFCs in 4–5 mo old mice. (F–J) IgG2c specific antibodies against dsDNA, histone, nucleosome, Sm/RNP, cardiolipin, and IgG2b-specific antibodies against nucleosome were measured through ELISA. (K–O) ELISPOT analysis of IgM-, IgG-, dsDNA-, histone-, and nucleosome-specific long-lived bone marrow AFCs at 4–5 months. Each circle represents an individual mouse. *p <0.05, **p <0.01, ***p <0.001.