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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Immunol. 2015 Mar 27;194(9):4362–4370. doi: 10.4049/jimmunol.1402384

Figure 5. Crk/CrkL DKO Tregs do not show decreased suppressive function compared to WT Tregs.

Figure 5

(A) Representative flow cytometric plots and (B) the fraction of Tregs (out of CD4+ T cells) from the thymus and spleen of WT and Crk/CrkL DKO Tregs are shown. (C) The expression of CTLA-4, CD25, GITR, and CD44 on Tregs from WT and Crk/CrkL DKO Tregs is shown. (D) CFSE-labeled splenic CD4+Foxp3− Tconvs were cultured with either WT or Crk/CrkL DKO Tregs for four days in the presence of irradiated T cell-depleted splenocytes and anti-CD3. Representative CFSE plots from four-day cultures lacking Tregs or containing a 1:2 or 1:1 Tconv to Treg ratio of either WT or Crk/CrkL DKO Tregs are shown (top). A plot of the division index of Tconvs at various Treg:Tconv ratios are shown (middle). (E) The division index of Tconvs at Tconv:Treg ratios of 1:2 and 1:1 were normalized to the baseline Tconv division (no Tregs) for each experiment and represented as mean % suppression ± SEM (n = 4 mice total).