Cone photoreceptor-specific deletion of PIK3CA, a pan-p110α catalytic subunit of PI3K, was performed by cross-breeding floxed p110α mice to cone-specific Cre mice (A). PCR diagnostic for cone-opsin cre (B), and floxed p110α and WT genes (C) using mouse tail DNA samples. Immunohistochemical analysis of Cre recombinase immunolabeling in p110α KO and WT control retinas harvested from littermates. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer. Red indicates Cre-positive cone cells. Labeling of blood vessels (*) is non-specific.