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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Immunol. 2015 Mar 30;194(9):4555–4566. doi: 10.4049/jimmunol.1402734

Figure 2. SNX17 knockdown in CD4+ T cells leads to decreased TCR and LFA-1 surface expression.

Figure 2

(A) Top, protein lysates prepared from lentiviral vector shControl and shSNX17 Jurkat T cells were immunoblotted for SNX17 and γ-Tubulin (as a loading control). Bottom, protein lysates prepared from siRNA Control and siSNX17 primary human CD4+ T cells were immunoblotted for SNX17 and γ-Tubulin (as a loading control). (B) shControl (black line) and shSNX17 (gray line) Jurkat T cells were antibody stained for surface TCRαβ and CD11a and analyzed using flow cytometry. Histogram and bar graphs are representative of eight separate experiments. (C) Unstimulated and anti-CD3/CD28 stimulated siControl (black) and siSNX17 (gray) primary human CD4+ T cells were surfaced labeled for TCRαβ (left), CD11a (center), and CD18 (right) were analyzed using flow cytometry. Histograms and graphs are representative of four separate experiments. Bars represent mean ± SEM. Horizontal lines indicate statistical comparison between groups, *p≤0.05, **p≤0.01 and ***p≤0.0005.