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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Immunol. 2015 Mar 30;194(9):4555–4566. doi: 10.4049/jimmunol.1402734

Figure 3. SNX17 knockdown leads to decreased conjugate formation and activation.

Figure 3

(A) Diagram of the Conjugate assay protocol. (B) Percent conjugates were measured and contour plots made for shControl (black box) and shSNX17 (white box) Jurkat T cells in culture with +SEE loaded Raji cells as shown in A. (C) ShControl (black) and shSNX17 (white) Jurkat T cells in the conjugate assay were antibody labeled for CD69 and permeabilized and phospho-labeled for pZAP-70 and analyzed using flow cytometry. B and C are representative of four separate experiments. Bars represent mean ± SEM. *p≤0.0005.