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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: J Immunol. 2015 Mar 30;194(9):4555–4566. doi: 10.4049/jimmunol.1402734

Figure 6. SNX17 localizes with the TCR at the immune synapse.

Figure 6

(A) Primary human CD4+ T cells were placed in co-culture with CMAC7 stained SE cocktail loaded Raji cells then fixed and labeled with antibodies against TCR and SNX17 for IF. (B) Line intensities profiles were created from the yellow line (bottom graph) and the dotted line (top graph) in images from A using FIJI. (C) Images from A were analyzed for the co-localization of SNX17 with the TCR at the T cell-Raji interface and at the T cell distal membrane using Pearson’s co-localization coefficient in ZEN. Zoomed images are demarcated by the white box in the adjacent image. Differential interference contrast (DIC) image used to demarcate the outline of the cell. For A and C, >20 T cell-Raji conjugates were imaged and analyzed from each of 3 separate imaging experiments. Images were collected with 100x oil objective. Scale bars, 10 μm. Bars represent mean ± SEM. Horizontal lines indicate statistical comparison between groups, *p≤0.05.