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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Neurobiol Dis. 2015 Mar 11;77:191–203. doi: 10.1016/j.nbd.2015.03.005

Figure 5.

Figure 5

Representative photomicrographs of striatal DARPP-32 staining in young (A, D, G), middle-aged (B, E, H), and aged (C, F, I) rats with intrastriatal DA neuron grafts (*asterisk). These images show that the graft protocol used resulted in an equivalent, relatively small area of disruption of DARPP-32 positive neurons across the various host age groups despite varying degrees of TH+ graft number and infiltration into the host striatum. Quantification data of the percent of striatal volume devoid of DARPP-32+ immunoreactivity showed no age-related difference in the volume of striatum staining for DARPP-32 (VM grafted rats: young 99.85% ± 0.86; middle-aged 98.30% ± 0.57; aged 96.93% ± 0.473; % of striatal volume with DARPP-32-ir; F(2,36.)=1.18, p > 0.05) and equivalent to the volume of DARPP-32 staining in sham-grafted rats (sham-grafted rats: young 99.97% ± 0.75; middle-aged 99.95% ± 0.53; age 99.97% ± 0.669). Scale bars represent 100 µm.