Fig. 3.

NC15A-meidated Gag processing efficiency is significantly enhanced following removal of MA. 293T cells were transfected with designated constructs. At 4 h post-transfection, equal amounts of cells were plated on three dishes. Cell were collected at 8, 16 or 24 h post-transfection and subjected to Western immunoblotting. Cellular Pr55^gag and p24^gag levels were quantified by scanning immunoblot band densities. Ratios of p24^gag to Pr55^gag or to mutant Gag precursor were determined for each mutant and normalized to those of the wt in parallel experiments. Values were derived from three independent experiments. Bars indicate standard deviations. ^∗p < 0.05.