Fig. 7.

Relative levels of virus-associated RNA. 293T cells were transfected with PR-inactivated (D25) versions of the indicated constructs. Virus-containing supernatants were pelleted and resuspended in PBS buffer. About 40% of the resulting suspensions were subjected to Western immunoblot analysis, and the remaining suspensions were subjected to RNA extraction and quantification as described in Section 2. Ratios of RNA amounts to Gag protein levels obtained by quantifying immunoblot band densities were determined for each mutant and normalized to those of wt (D25) in parallel experiments. Bars indicate standard deviations. ^∗p < 0.05.