TABLE 2.
Inhibition of E. coli macromolecular synthesis pathways
| Compound | Target | Incorporation IC50 (μg/ml)a |
||||
|---|---|---|---|---|---|---|
| Protein | Cell wall | Fatty acid | RNA | DNA | ||
| [14C]leucine | [3H]DAPb | [14C]acetic acid | [3H]uridine | [3H]thymidine | ||
| Erythromycin | Protein synthesis | 30 | >256 | >256 | >256 | >256 |
| Ampicillin | Cell wall synthesis | >256 | 16 | >256 | >256 | >256 |
| Triclosan | Fatty acid synthesis | >256 | 1.5 | 0.0156 | 2 | 2 |
| Rifampin | Transcription | 64 | >256 | >256 | 64 | >256 |
| Ciprofloxacin | DNA replication | 30 | >256 | >256 | 1 | 0.02 |
| Carbonyl cyanide m-chlorophenylhydrazone (CCCP) | Membrane potential | 50 | 0.15 | 0.07 | 0.25 | 0.3 |
| Compound 1 | Cell wall synthesis | >256 | 20 | 0.4c | >256 | >256 |
| Compound 2 | Cell wall synthesis | >64 | 16d | >64 | >64 | >64 |
a
Incorporation of radiolabeled precursors was measured in E. coli ΔtolC ΔlysA. IC50, 50% inhibitory concentration.
b
DAP, diaminopimelic acid.
c
The IC50 is based on a maximum inhibition of <40%.
d
The IC50 is based on a maximum inhibition of <42% due to the compound solubility.