TABLE 1.
List of N. gonorrhoeae strains used
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| FA1090 | Background strain | J. Cannon |
| Phase-variable parent | FA1090 recA6::Tetr | 37 |
| Phase-variable ΔmutS mutant | FA1090 recA6 mutS::Ermr | 32 |
| Phase-variable Kanr parent | FA1090 recA6 igr1584::Kanr | This study |
| Phase-variable mutS-F32A mutant | FA1090 recA6 mutS-F32A igr1584::Kanr | This study |
| Phase-variable ΔmutL mutant | FA1090 recA6 ΔmutL | 32 |
| Tn9/G4mtAvd-1 | FA1090 recA6 Avd-1 | 13 |
| Phase-variable ΔpilE mutant | FA1090 recA6 ΔpilE | A. Criss |
| FA7458-1A | FA1090 A23a ΔpilC2 pilC1PL | 47 |
| Parent | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat | This study |
| Kanr parent | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat igr1584::Kanr | This study |
| ΔmutS mutant | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS::Ermr | This study |
| Kanr ΔmutS mutant | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS::Ermr igr1584::Kanr | This study |
| mutS-F32A mutant | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS-F32A igr1584::Kanr | This study |
| mutS-S661A mutant | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS-S661A igr1584::Kanr | This study |
| Complemented parent | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS-NICS | This study |
| ΔmutL mutant | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat ΔmutL | This study |
| ΔmutS ΔmutL mutant | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS::Ermr ΔmutL | This study |
| Complemented ΔmutS strain | FA1090 A23a ΔpilC2 pilC1PL recA6 RS1::cat mutS::Ermr mutS-NICS | This study |
| Phase-variable ruvB recG mutant | FA1090 ruvB::Ermr recG::Kanr recA6 | 49 |
| Phase-variable ruvB mutant | FA1090 ruvB::Ermr recA6 | 49 |
| ruvB mutant | FA1090 ruvB::Ermr recA6 pilC1PL | This study |
| ruvB recG mutant | FA1090 ruvB::Ermr recG::Kanr recA6 pilC1PL | This study |
| ruvB recG ΔmutS mutant | FA1090 ruvB::Ermr recG::Kanr recA6 pilC1PL mutS::Ermr | This study |
| ruvB recG mutS-F32A mutant | FA1090 ruvB::Ermr recG::Kanr recA6 pilC1PL mutS-F32A igr1584::Kanr | This study |