FIG 10.

Peptide 6-R can compete with 5-R in the agrB-agrD system. Final 100 or 250 μM concentrations of peptide 6-R, along with a 100 μM concentration of peptide 5-R, were added into fresh TGY broth, followed by inoculation of that broth with CN1795agrBko. The culture was anaerobically incubated overnight at 37°C. Culture supernatant was collected for detection of CPB using a Western blot assay. Cultures of wild-type CN1795, CN1795agrBko, CN1795agrBko with the addition of 100 μM 5-R, and CN1795agrBko with the presence of 100 μM 6-R were tested similarly as controls.