FIG 4.

Time course study of CPB production by CN1795agrBko and CN3685agrBko in the presence of peptides 5-R and 5-L. Signaling peptide (100 μM) was added to the TGY broth followed by the inoculation of bacteria at 37°C. Samples were collected at 3 h, 6 h, and 16 h (overnight), and those supernatants were then used for Western blot detection for CPB production. The kinetics of natural CPB production by TGY cultures of wild-type CN3685 or CN1795, without added peptide, are shown for comparison. All experiments were repeated three times.